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Pshuttle-gfp

WebMay 30, 2024 · We constructed three new adenoviral vectors based on the shuttle plasmid pShuttle (Stratagene, La Jolla, CA, USA). The vectors (pAd shRNA/mU6, pAd shRNA/H1, and pAd shRNA/U6) all contain an shRNA expression cassette and an enhanced GFP (EGFP) marker, and all three constructs have the same cloning sites . The shRNA cassette is … WebGFP) and the shuttle vectors (pShuttle) were digested with NotI and KpnI. The 412bp (U6-Acrp30 cDNA) and 6.6 kb (pShuttle) frag-ments were collected and ligated. The shuttle plasmids (pShut-tle-U6-Acrp30 and pShuttle-GFP) were linearized by Pme I and transformed into BJ5183-AD-1 cells (harbor pAdEasy-1) by elec-troporation.

Adenovirus-based short hairpin RNA vectors containing ... - BioTechniques

WebMethods: From pcDNA3.1-NELL1, NELL1 gene sequence was obtained, then NELL1 gene was subcloned into pShuttle-GFP-CMV (-)TEMP vector which was subsequently digested with enzyme and inserted into pAdxsi vector to package the recombinant adenovirus vector (pAdxsi-GFP-NELL1). WebNov 2, 2015 · The Ad vector pShuttle-CMV (Stratagene) was rendered replication defective by deletion of the E1 and E 3 genes [ 23 ]. The GFP coding sequence was amplified from the pEGFP-N1 vector (Clontech) using the primer pair 5′-ATGGTGAGCAAGGGCGAG-3′ and 5′-TTACTTGTACAGCTCGTCC-3′. how can i play stray https://youin-ele.com

AdEasy Adenoviral Vector System - Agilent …

WebDec 15, 2024 · In Brief, pShuttle-GFP-Gas6 was constructed by cloning the target gene Gas6 into pShuttle-GFP-CMV. Then, the pShuttle-GFP-Gas6 was transferred into the recombinant adenovirus frame vector of pADxsi after sequence confirmation. WebMar 1, 2024 · BglII + KpnI digestion to identify pShuttle-GFP-CMV(-)-PD-L1. Construction and Identification of PD-L1 Recombinant Adenovirus Skeleton Plasmid The successfully … WebRecombinant adenoviruses are a versatile tool for gene delivery and expression. Choose pAdEasy-1 vector or any of these three shuttle vectors, pShuttle, pShuttle-CMV, or … how can i play the game slingo for free

Addgene: pShuttle-CMV-GFP

Category:Differentiation of PDX1 gene-modified human umbilical cord …

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Pshuttle-gfp

Generate recombinant adenoviruses for gene expression

WebPlasmid pShuttle-CMV-GFP from Dr. Oskar Laur's lab contains the insert CMV promoter-EGFP. This plasmid is available through Addgene. Recombine plasmids from the shuttle vectors (pShuttle, pShuttle-CMV, pAdTrack, … WebpShuttle-IRES-hrGFP-2. Plasmid type:Adenovirus systemCopy Number:Low copyPromoter:CMVCloning Method:Multiple cloning sites,restriction …

Pshuttle-gfp

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Webany of the four shuttle vectors (pShuttle-CMV, pShuttle, pShuttle-IRES-hrGFP-1 (Catalog #240081) or pShuttle-IRES-hrGFP-2 (Catalog #240082). Once constructed, the shuttle … WebpShuttle-IRES-hrGFP-1 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially …

WebVector Name pShuttle-IRES-hrGFP-1; Specifications/Features Vector Size: 8.9 kb. Add to Compare List. Agilent Technologies. 5301 Stevens Creek Blvd Santa Clara, California 95051. United States Phone: +1 877-424-4536. Company … WebpShuttle-IRES-hrGFP-2 $280.00 Current Reviews: 0 Add Your Review Units in Stock : 20 QTY : MESSAGE TO US Description Reviews pShuttle-IRES-hrGFP-2 PVTY00778 2ug pShuttle-IRES-hrGFP-2 Description

WebpSuper pSuper retro GFP-neo pSupeior puro pBabe puro pCMV-Gag-pol pCMV-VSV-G pCL Ampho pSuper retro puro pLVX-shRNA1 pLVX-shRNA2 pSicoR pSicoR pGK puro pLentilox 3.7 pLKO.1 puro PLKO.1 pLKO.3G pTet-PLKO-puro pPRIME-TET-GFP-FF3 pPRIME-TREX-GFP-FF3 pSIH1-H1-CoGFP pGIPZ empty pGIPZ control pTRIPZ empty pTRIPZ control pLVX … WebProduct Specs. Item pShuttle-IRES-hrGFP-2. Company Agilent Technologies. Price Supplier Page View Company Product Page. Catalog Number 240082. Type Adenoviral Vector. …

WebDec 1, 2009 · The formed plasmids, pShuttle-GFP-TPE and pShuttle-GFP-D19K, respectively, were the final shuttles. These two plasmids were recombined with pAdEasy-1 to generate oncolytic Ad plasmids carrying the GFP gene. 3.2. Armed oncolytic Ads were rescued in packaging cells Ads were rescued in 293 cells by transfection of PacI-linearized Ad …

WebA GGGGCC hexanucleotide repeat expansion in intron 1 of chromosome 9 open reading frame 72 (C9ORF72) gene is the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal ... how many people do mind helpWebpShuttle pShuttle CMV pRL CMV pCMV-HA pVAX1 pVAST pCI pCI neo+ pCMV-myc pflag-CMV2 pcDNA3.1 + pcDNA3.1 zeo+ pcDNA3.1 neo+ pcDNA3.1 MycHisA pcDNA3.1 MycHisB pcDNA3.1-HBV genome pcDNA3-mRFP Hsp70-pcDNA3.1 pcDNA 4 Max B psuper GFP neo psuper Retro GFP neo pSilencer4.1CMV puro+ pSilencer4.1CM neo+ psuper GFP neo … how can i play the c f an g on basshttp://www.labbase.net/Supply/SupplyItems-4229347.html how can i play swf files pcWebDec 1, 2009 · The formed plasmids, pShuttle-GFP-TPE and pShuttle-GFP-D19K, respectively, were the final shuttles. These two plasmids were recombined with pAdEasy-1 to generate … how can i play tsukihimeWebAug 14, 2015 · zt492 pShuttle-IRES-hrGFP2 Stratagene 腺病毒系统. zt493 pacAd5 CMV Cellbiolabs 腺病毒系统. zt494 pacAd5 9.2-100 Cell biolabs 腺病毒系统. zt495 pacAd5 CMV-GFP Cell biolabs 腺病毒系统. zt496 pDC311 Microbix 腺病毒系统. zt497 pDC312 Microbix 腺 … how can i play the papas games without flashWebSep 1, 2013 · Results: The sequence of hVEGF165 in pShuttle-GFP-hVEGF165 plasmid was confirmed by double-enzyme cleavage method and sequencing. hVEGF165 was highly … how many people does the shoe holdWebby SinoGenoMax Co, Ltd (Beijing, China). Briefly, pShuttle-GFP-CTRP3 was constructed by cloning the target gene CTRP3 into pShuttle-GFP-CMV. After sequence confirmation, … how can i play ultimate soccer manager 2