WebMay 30, 2024 · We constructed three new adenoviral vectors based on the shuttle plasmid pShuttle (Stratagene, La Jolla, CA, USA). The vectors (pAd shRNA/mU6, pAd shRNA/H1, and pAd shRNA/U6) all contain an shRNA expression cassette and an enhanced GFP (EGFP) marker, and all three constructs have the same cloning sites . The shRNA cassette is … WebGFP) and the shuttle vectors (pShuttle) were digested with NotI and KpnI. The 412bp (U6-Acrp30 cDNA) and 6.6 kb (pShuttle) frag-ments were collected and ligated. The shuttle plasmids (pShut-tle-U6-Acrp30 and pShuttle-GFP) were linearized by Pme I and transformed into BJ5183-AD-1 cells (harbor pAdEasy-1) by elec-troporation.
Adenovirus-based short hairpin RNA vectors containing ... - BioTechniques
WebMethods: From pcDNA3.1-NELL1, NELL1 gene sequence was obtained, then NELL1 gene was subcloned into pShuttle-GFP-CMV (-)TEMP vector which was subsequently digested with enzyme and inserted into pAdxsi vector to package the recombinant adenovirus vector (pAdxsi-GFP-NELL1). WebNov 2, 2015 · The Ad vector pShuttle-CMV (Stratagene) was rendered replication defective by deletion of the E1 and E 3 genes [ 23 ]. The GFP coding sequence was amplified from the pEGFP-N1 vector (Clontech) using the primer pair 5′-ATGGTGAGCAAGGGCGAG-3′ and 5′-TTACTTGTACAGCTCGTCC-3′. how can i play stray
AdEasy Adenoviral Vector System - Agilent …
WebDec 15, 2024 · In Brief, pShuttle-GFP-Gas6 was constructed by cloning the target gene Gas6 into pShuttle-GFP-CMV. Then, the pShuttle-GFP-Gas6 was transferred into the recombinant adenovirus frame vector of pADxsi after sequence confirmation. WebMar 1, 2024 · BglII + KpnI digestion to identify pShuttle-GFP-CMV(-)-PD-L1. Construction and Identification of PD-L1 Recombinant Adenovirus Skeleton Plasmid The successfully … WebRecombinant adenoviruses are a versatile tool for gene delivery and expression. Choose pAdEasy-1 vector or any of these three shuttle vectors, pShuttle, pShuttle-CMV, or … how can i play the game slingo for free