Phospholipidosis assay
WebCytotoxicity Assays. Cytotoxicity is the degree to which a substance can cause damage to a cell. A substance or process that causes cell damage or death is referred to as cytotoxic, "cyto" meaning cell and "toxic" meaning poison. Cells exposed to cytotoxic compounds may undergo necrosis (uncontrolled cell death), apoptosis (programmed cell ... WebMar 5, 2015 · Synthesis and phospholipidosis effect of a series of cationic amphiphilic compounds: a case study to evaluate in silico and in vitro assays Article Full-text available
Phospholipidosis assay
Did you know?
WebSep 3, 2013 · In this study, a cell-based phospholipidosis assay has been developed using the LipidTOX Red reagent in HepG2 cells and miniaturized into a 1536-well plate format. To validate this assay for high ... WebJun 12, 2007 · Using an expanded set of compounds, we show that this assay correctly identified 100% of PLD-positive and -negative compounds. Dose-dependent increases in intracellular fluorescent phospholipid accumulation were observed. We found that this assay was less time consuming, more sensitive, and higher throughput than gene expression …
WebPhospholipidosis (PL) is lysosomal storage disorder characterized by the excessive accumulation of intracellular phospholipids (Figure 1). The mechanism of phospholipidosis is still unclear today, but it is known that phospholipidosis is commonly caused by the intake of a special group of drugs containing a hydrophobic ring/chain and a hydrophilic amine … WebEchelon’s LPLA2 Inhibitor Screen is a high throughput assay designed to screen drugs abilitiy to inhibit LPLA2 activity in vitro, a potential predictor of drug-induced phospholipidosis. Sample Volume: 1 μL. Sample Number: 128 Samples (in triplicates)/384-well plate. Echelon’s LPLA2 Inhibitor Screen (K-7000I) is designed to assay a drug’s ...
WebTo identify compounds that may induce phospholipidosis early in the discovery process, we have developed a predictive fluorescent cell-based assay amenable to automated high … WebAug 2010 - Oct 20144 years 3 months. Jalandhar,Punjab,India. The active area of research at University was Metabolic disorder (Diabetes).I was involved in in-vitro selection & characterization, target validation for metabolic targets, assay development and condition optimization, its high throughput screening, selection of hits among phyto ...
WebMar 1, 2024 · This assay has been optimized and validated in HepG2 and HepRG cells, and miniaturized into a 1536-well plate, thus can be used for high-throughput screening (HTS) to identify chemical compounds...
Web+ selectively discriminates phospholipidosis from steatosis assay ready HepG2 cells assay ready HepG2 cells have an instantly high viability, low debris and attach within 24 hours. drug induced phospholipidosis assay ready HepG2 were treated for 48 hours with Sertraline, a potent inductor of phospholi-pidosis, and Cyclosporine A for negative ... mount pleasant sc veterinary clinicsWebMar 1, 2024 · The EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large … mount pleasant sc zoning ordinanceWebJul 2, 2008 · In vitro screening assays were developed in HepG2 cells, rat primary hepatocytes, and rhesus monkey hepatocytes using the fluorescent-labeled phospholipid probe N - (7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl- sn -glycero-3-phosphoethanolamine (NBD-PE) or Nile Red lipid stain. mount pleasant sheltered housingWebIn this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H 2 … heartland ranch in hudson canadaWebPhospholipidosis is a lysosomal storage disorder characterized by the excess accumulation of phospholipids in tissues, often stimulated by drugs. Drug induced hepatotoxicity is a … mount pleasant sc wedding venueWebLC-MS lipid profiling of HepG2 cells is proposed as an in vitro assay to study and anticipate phospholipidosis and steatosis. Cells with and without preincubation with a mixture of … heartland ranch in canadaWebSuch assays rely heavily on the development prospective assays aim to predict toxicities that could lead to the ter- of human physiologically relevant model systems in combination with mination of a drug candidate or an entire development programme, appropriate toxicity end points. and bespoke retrospective assays are used for issue resolution ... heartland ranch tv show