Hiscript qrt supermix for qpcr +gdna wiper
WebbHiscript III RT SuperMix for PCR (+gDNA wiper) is an upgraded vesion of Hiscript III Q RT Supermix for GPCR (+gDNA wiper), induding Hiscript III Reverse Transcriptase, … Webb13 juni 2024 · Transcriptome and qRT-PCR analyses revealed that ZjBAMs respond positively to elevated temperature and drought stress. Specifically, ZjBAM1, ZjBAM2, ZjBAM5, and ZjBAM6 are significantly upregulated in response to severe drought.
Hiscript qrt supermix for qpcr +gdna wiper
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Webb27 apr. 2024 · The cDNAs were synthesized with HiScript qRT Supermix for qPCR (+gDNA wiper; R123-01; Vazyme Biotech, Nanjing, China). The RT-qPCR was performed using SYBR Green (TaKaRa Bio Inc., Kusatsu, Shiga, Japan) on the ABI PRISM 7700 System (Applied Biosystems, Foster City, CA, USA). ACTIN2 was used as Webb10 apr. 2024 · Total RNA was isolated from cells by RNA extraction kit (Vazyme, China), which was reversely transcribed according to the instructions of the HiScript II Q RT SuperMix for qPCR (+ gDNA WIper) (Vazyme, China) reverse transcription kit. qRT-PCR analysis was conducted by SYBR Green (TaKaRa, Japan) in StepOnePlus™ Real …
WebbThe HiScript II Q RT SuperMix for qPCR (+gDNA wiper) is specially designed for two-step qRT-PCR. The residual genomic DNA in RNA template can be removed rapidly … WebbHiScript® III RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript® II Q RT SuperMix for qPCR (+gDNA wiper), including a new generation of reverse …
WebbTotal RNA was isolated from cells using Trizol reagent (Invitrogen). The first chain cDNA was synthesized using HiScript II Q-RT SuperMix for qPCR (+gDNA wiper, Vanzym, China). RT-PCR analyses were performed on a LightCycler 96 detection system (Roche) according to the manufacturer’s instructions. WebbThe 4 × gDNA wiper Mix in the product can rapidly remove the residual genomic DNA contamination and ensure more reliable subsequent quantitative results. 5 × HiScript II …
Webb18 juni 2024 · Reverse transcription was performed using HiScript II QRT SuperMix for qPCR (gDNA wiper) (Vazyme Biotech). Real-time PCR was performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme Biotech in a LightCycler® 96 Real-time system (Roche). All primers are listed in Supplementary Table 2. 3. Results and …
Webb22 nov. 2024 · Reverse transcription was conducted with 1 g of total RNA sample in a 20 L reaction mixture using the HiScriptfiII QRT SuperMix for qPCR (+gDNA wiper) (Vazyme, Nanjing, China) to synthesize cDNA. All the operations followed the manufacturer’s instructions. Reactions without reverse transcriptase or a template were both used as … cap anson baseball statsWebb6 jan. 2024 · Total RNA was extracted from 0.1 g of bud tissue using an RNAprep Pure Plant Kit (TianGen,Beijing,China)according to the manufacturer’s instructions.First-strand cDNA was then produced using HiScript Q RT SuperMix for qPCR (+gDNA-wiper) (Vazyme,Nanjing,China)according to the manufacturer’s instructions.qPCR was … cap antalya turchiaWebbThe HiScript II Q RT SuperMix for qPCR (+gDNA wiper) is specially designed for 2-step qRT-PCR. The residual genomic DNA in RNA template can be removed rapidly and … british heart foundation lottery resultsWebb13 apr. 2024 · cDNA合成の効率をさらに向上させ、2段階のRT-qPCR検出に最適です。 特長 HiScript® III逆転写酵素、耐熱性DNase、バッファー及びランダム/オリゴdTミックスプライマーがプレミックス ゲノムDNAの除去と逆転写を同時に行うことができ、得られたcDNAは長期間安定して保存可能 ゲノムDNAのコンタミネーションやRNAの分解を大 … cap and trim fenceWebbHiscript® III RT SuperMix for qPCR (+gDNA wiper) R323 Price: $435.00 Catalog #: R323-01 * Package Size: 100 rxn Quantity: Buy in bulk and save For larger amounts, … british heart foundation lottery ukWebbThe 4 × gDNA wiper Mix, 5 × HiScript III qRT SuperMix and 5 × No RT Control Mix contain high concentration of glycerol. Please centrifuge briefly and pipette up and … british heart foundation magazine onlineWebb9 aug. 2024 · Transfection was performed using Fugene HD Transfection Reagent (Promega Biotechnology Co., Ltd., Beijing), Total RNA Extraction Reagent TRIzol (15596-026, Invitrogen, Waltham, MA, USA), a Reverse Transcription Kit (Vazyme, Hiscript II qRT SuperMix for qPCR (gDNA wiper) R223-01 China), and qPCR (Vazyme, ChamQ … c.a.papaellinas group