WebFlowJo is your biggest fan and strives to be an outstanding source of support. We’re here to help you accelerate routine phenotyping, take your immunology research to the next level, and get you from data to results―one cell at a time. Accelerate your discovery with the leading platform for single-cell flow cytometry analysis. Tackle your ... WebAug 9, 2024 · You could calculate new compensation with the new controls and apply it to the old fully stained tubes, but please be aware that this situation is not ideal and there can be complications to running controls and samples on different days (see more about this in point 1 here). If you determined that the polymer dyes were sticking together then ...
Demystifying Compensation in FlowJo® - The Daily …
WebIn FlowJo, choose the appropriate gate when defining transformation Data provided by Jeff Pufnock, 061307, CD8 minus FMO ... – Apply compensation to comp samples. Does compensation matrix need to be re-calculated or is the comp matrix not working for the test samples, but ok for the comp samples. ... WebMar 31, 2024 · Part three of a series on data analysis using FlowJo. Demonstrating how to use FMO controls for gating. calvin klein allianz
Compensation - FlowJo Documentation FlowJo …
WebFluorescence compensation. Emission spectra of two fluorophores commonly used in flow cytometry, FITC and PE are shown. Also shown is a graphical representation of two commonly used filters, 525/50 and … WebThis is a simple example showing how to do three-color compensation in FlowJo. There are several steps involved. Set a gate for the positive and negative populations for each singly-stained sample. Bring up the Compensation Definition dialog window, or Compensation Wizard. Drag each of the positive and negative populations from the … Web3-5 years’ experience as a Compensation Analyst. Bachelor’s Degree, or equivalent experience, required. Prior experience with Workday preferred. Previous experience with … calvin klein all in one