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combination of sb431542, chir9901, and bpv as a novel
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Use of organoids to study regenerative responses to intestinal damage ...
WebBackground: Small molecule compounds have been well recognized for their promising power in generation, expansion and maintenance of embryonic or adult stem cells. The aim of this study was to identify a novel combination … WebDec 13, 2024 · Addition of CHIR9901, a GSK-3 inhibitor, to human intestinal cultures significantly improved survival and growth after radiation ( 65) via selective blockade of p53-induced apoptosis in the ISC. These in vitro organoid studies suggest that p53 regulation may be important to radioprotective responses. WebMay 15, 2024 · ES cells were cultured on gelatin-coated dishes in ES cell media containing 1.5 µM CHIR9901 (GSK3 inhibitor) for several passages to remove feeder cells. ES cells were passed by washing with PBS, and dissociating with trypsin. Extracted molecule genomic DNA Extraction protocol Cells were crosslinked with formaldehyde and … the original factory shop mablethorpe