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Atga2ox8基因过表达载体构建及遗传转化植株表型分析

WebAtGA2ox7 and AtGA2ox8 are more similar to each other than to other proteins encoded in the Arabidopsis genome, indicating that they may constitute a separate class of GA-modifying enzymes. Indeed, enzymatic assays demonstrated that AtGA2ox7 and AtGA2ox8 both perform the same GA modification: 2beta-hydroxylation of C20-GAs but not of C19 … WebApr 30, 2024 · 为研究自噬相关基因 ATG8g 在香蕉中的潜在功能，以及在过表达植株中对植株形态发育的影响，本研究通过农杆菌转化法获得 MaATG8g 拟南芥过表达植株。 结果 …

The Multifunctional Dioxygenases of Gibberellin Synthesis - OUP …

WebJun 14, 2024 · We compared the expression changes of AtGA2ox7 or AtGA2ox8 between wild-type (WT) and zma-unmiR4-overexpressing (zma-unmiR4 OE) plants (Figure 3b). As shown in Figure 3c, AtGA2ox7 was significantly down-regulated while AtGA2ox8 displayed no obvious changes in both zma-unmiR4 overexpressors, suggesting that AtGA2ox7 … WebDec 14, 2010 · Gibberellins (GAs) are endogenous hormones that play an important role in regulating plant stature by increasing cell division and promoting seed germination. The GA2-oxidase gene from Arabidopsis thaliana (AtGA2ox8) was introduced into Brassica napus L. by Agrobacterium-mediated floral-dip transformation with the aim of decreasing … cream woes have inspired memes mockery https://youin-ele.com

Expression of gibberellin 2-oxidase 4 from Arabidopsis under the ...

WebWe identified two genes in Arabidopsis (AtGA2ox7 and AtGA2ox8), using an activation-tagging mutant screen, that encode 2beta-hydroxylases. GA levels in both activation … WebUse Bio-Rad's PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed. WebOverexpression of both AtGA2ox7 and AtGA2ox8 caused dwarfing ( Figures 1C and 1D), indicating that the substrates for these enzymes are required for normal elongation … cream with hyaluronic acid boots

CRISPR-Based Assessment of Gene Specialization in the …

Category:Full article: Analysis of regulatory elements in GA2ox, GA3ox and ...

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Atga2ox8基因过表达载体构建及遗传转化植株表型分析

ATGA2OX8 - PrimePCR Assay and Template Life Science Bio …

WebJan 27, 2024 · 研究人员对小麦ABS3和ATG8同源蛋白的研究发现，ATG8-ABS3互作调控衰老的范式在双子叶和单子叶植物中都存在。. 基于ABS3亚家族基因促进衰老的途径与自 …

Atga2ox8基因过表达载体构建及遗传转化植株表型分析

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WebAug 1, 2024 · GA2-oxidases (GA2oxs) are a class of 2-oxoglutarate-dependent dioxygenases that regulate the deactivation of bioactive GAs. In this study, we investigated the phylogeny and domain structures of the seven GA2ox genes present in the Arabidopsis thaliana genome. Web过表达载体主要是将目的基因的编码区（CDS）构建到相应的质粒或者病毒载体中，达到在目的基因过量表达的作用。 过表达载体的主要元件：Promoter—gene—linker—Fluorescent gene—抗药筛选gene。 花椰菜花叶病毒 (CaMV) 35S是组成型启动子，具多种顺式作用元件。 其转录起始位点上游－343～－46bp是转录增强区，－343～－208和－208～－90bp …

WebApr 12, 2024 · Los Angeles , city, seat of Los Angeles county, southern California, U.S. It is the second most populous city and metropolitan area (after New York City) in the United … WebJan 1, 2024 · We found that CsGA2ox8, a gene whose product catalyzes the deactivation of bioactive GAs, was significantly upregulated in treatment X1 compared to treatment X2. This expression difference was...

WebJul 3, 2010 · Abstract Gibberellin 2-oxidase (GA 2-oxidase) plays very important roles in plant growth and development. In this study, the AtGA2ox8 gene, derived from … WebAtGA2ox7 and AtGA2ox8 are more similar to each other than to other proteins encoded in the Arabidopsis genome, indicating that they may constitute a separate class of GA …

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WebThe two GA2ox genes – AtGA2ox8 and AtGA2ox2 – were chosen because the enzymes that they encode catabolize early precursors of GA (C 20 -GA) or late precursors and bioactive GAs (C 19 -GA), respectively ( Schomburg et al., … dmv michigan driving test 2022WebApr 11, 2024 · Jo-Anne Theresa Glassock. July 4, 1946 - March 20, 2024 Dearly beloved Jo-Anne Theresa Glassock passed away on March 20, at the age of 76, in Laguna … dmv michigan test 2021WebNov 14, 2014 · The tblastn program was run to identify the homologous gene sequences in all available switchgrass expressed sequence tag (EST) databases using the amino acid sequences of AtGA2ox8 , OsGA2ox5 (LOC_Os07g01340.1), OsGA2ox6 (LOC_Os04g44150.1) and OsGA2ox9 (LOC_Os02g41954.1). Phylogenetic trees and … dmv michigan lost titleWebOct 30, 2024 · Key message Using a fixed RIL population derived from a widely used foxtail millet backbone breeding line and an elite cultivar, we constructed a high-density bin map and identified six novel multi-environment effect QTLs and seven candidate genes for dwarf phenotype. Abstract Plant height is an important trait that determines tradeoffs between … cream with vitamin k oxideWebJul 13, 2024 · Both genes encode enzymes that have functional similarities to AtGA2ox7 and AtGA2ox8, which are class III GA 2-oxidases that 2β-hydroxylate C 20 -GAs. Previously unknown for GA 2-oxidases, AtGA2ox9 performs 2α-hydroxylation of C 19 -GAs and harbors putative desaturating activity of C 20 -GAs. dmv micrographics use onlyWebJan 1, 2024 · The homolog of CsGA2ox8 in Arabidopsis is AtGA2ox8, whose product functions in hydroxylating the C 20-GA precursors GA 12 and GA 53 15. In this study, consistent with the results from overexpression of AtGA2ox8 15, GA 12 and GA 53 levels dramatically decreased in OE8.1, suggesting that CsGA2ox8 has a function similar to … cream with vitamin b and dWebATG8 is an ubiquitin-like protein which can be used as an autophagosome marker in yeast, metazoans, and plants (Fujioka et al., 2008; Kirisako et al., 1999 ), and Arabidopsis has … cream womens lined crocs uk